Figure 8.

MtDAMP pre-treatment results in impaired neutrophil glycolysis. (A) Neutrophils pre-treated for 1 h with 1 mM AICAR or vehicle control were stimulated for 3 h with 25 nM PMA, after which lactate concentrations were measured in cell-free supernatants. Data are mean ± SEM of 12 independent experiments. ^***p < 0.0001. (B) Comparison of lactate concentrations in supernatants collected from neutrophils pre-treated with 100 μg/ml mtDAMPs or vehicle-control and subsequently stimulated with 25 nM PMA for 1, 2, and 3 h. Data are mean ± SEM of 10 independent experiments. ^**p < 0.001, ^***p < 0.0001 vs. vehicle.