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. 2018 Aug 30;28(11):860–875. doi: 10.1093/glycob/cwy079

Fig. 1.

Fig. 1.

(A) Domain architecture of SrFucNaFLD. (B) Protein sequence of S. roseum alpha-l-fucosidase in FASTA format depicting the amino acid sequence of the domains – alpha-l-fucosidase (Fuc), NPCBM_assoc (Na) and F-type lectin domain (FLD) within the polypeptide. (C) Digestion of recombinant clone with NcoI and XhoI demonstrating fallout of SrFucNaFLD gene from pET-28a(+); Lane 1: DNA marker from NEB; Lane 2: Positive clone with SrFucNaFLD insert (D) Coomassie blue-stained SDS-polyacrylamide gel with imidazole eluate fractions of a Ni-NTA metal ion affinity column, showing purified SrFucNaFLD protein; Lane 1: Protein marker from NEB; Lane 2 and 3: purified SrFucNaFLD protein.