Figure 5.

I696N greatly increased the antibody susceptibility of J6/JFH1 retaining HVR1 against the neutralizing antibodies AR3A and AR5A, whereas I696T did not. First passages of the indicated viruses were subjected to a dilution series of antibodies AR3A (A and C) or AR5A (B and D), and neutralization was assessed and analyzed as described in Figure 1, except we used 4-parameter curve fitting to obtain a sigmoidal dose-response curve. Error bars represent standard errors of the mean. Independent neutralization experiments yielded similar results.