(A) Quantification of calcium on spiral scaffolds with various numbers of HA bilayers deposited and control adsorbed HA. A direct correlation is shown between a number of bilayers deposited and the total calcium content on scaffolds. (B) Cumulative percent calcium ion release showing the release profiles of spiral scaffolds with 0, 1, 3, and 5 bilayers of HA deposited using the layer-by-layer technique across 60 days. An initial burst release of calcium is seen in all three bilayer scaffolds, followed by a steady, sustained release from day 7–60 with a direct correlation between a number of bilayers and the cumulative amount of calcium ions released.