Table 2.
Antibodies, Dilutions, and Retrieval Buffers Used
| Antibody | Dilution | Retrieval Buffer | Species | Supplier | Number |
|---|---|---|---|---|---|
| CYP11A1 | 1:10,000 | TEG | Rabbit | Sigma | HPA016436 |
| CYP17A1 | 1:1500 | CIT | Rabbit | Abcam | Ab134910 |
| 3β-HSD1/2 | 1:6000 | TEG | Rabbit | Gift from Ian Mason | — |
| CYP21A2 | 1:6000 | TEG | Goat | Santa Cruz Biotechnology | Sc-48466 |
| CYP11B1 | 1:1000 | TEG | Mouse | Santa Cruz Biotechnology | Sc-374096 |
| SULT2A1 | 1:6000 | CIT | Rabbit | Sigma | HPA041487 |
| MC2R | 1:800 | TEG | Rabbit | Santa Cruz Biotechnology | Sc-13107 |
Antigen retrieval was conducted by pressure cooking of the sections in indicated retrieval buffer for 30 minutes in a decloaking chamber. TEG buffer: 10 mM Tris, 0.5 mM EGTA, pH 9.0; citrate (CIT) buffer: 10 mM, pH 6.0.