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. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: J Mol Biol. 2018 May 19;430(17):2695–2708. doi: 10.1016/j.jmb.2018.04.044

Figure 1. A3G/B chimeras reveal that a key nuclear localization determinant of A3B resides in the first 30 amino acids.

Figure 1.

(a) Alignment of A3B and A3G with known and predicted secondary structures (α-helices and β-strands) depicted above amino acid sequences. Arrows indicate chimeric junction locations with residue numbers annotated above and below the junctions, and shading indicates identical residues. (b) Representative images of 293T and HeLa cells transiently expressing the indicated full length C-terminally eGFP tagged chimeric proteins (10 𝜇m scale bar). (c) Quantification of localization patterns for the indicated constructs in 293T cells. Individual cells expressing the indicated chimeric proteins were scored and grouped into three categories based on their overall patterns of cellular fluorescence (n=25 per condition; Nuc., nuclear localization; WC, whole cell localization; Cyto., cytoplasmic localization; ns, indicates no significance; ***, p < 0.001 by unpaired student’s t-test).