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. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: J Mol Biol. 2018 May 19;430(17):2695–2708. doi: 10.1016/j.jmb.2018.04.044

Figure 4. Reciprocal chimeras implicate a second surface-exposed region in A3B nuclear localization.

Figure 4.

(a) Alignment of A3B and A3G amino acid sequences as described in Figure 1. (b) Representative images of 293T and HeLa cells transiently expressing the indicated full length C-terminally eGFP tagged chimeric proteins (10 𝜇m scale bar). (c) Quantification of localization patterns for the indicated amino acid substitution mutants (n=25 per condition; image quantification and statistical analysis as described in Fig. 1).