Table 1. Accumulated somatic mutations per sample in each terminal branch of all sequenced plant samples.
Species (reference genome size) |
Samples | Diameter of the trunk (cm) | Estimated age (years) | DNA source | Sequenced samples | Accumulated mutations | |
---|---|---|---|---|---|---|---|
Average observed | Normalized rate (× 10−9 per bp per year)a | ||||||
P. mira (225 Mb) |
G1b | 207 | 600 | Leaf | 32 | 12.7 | 0.08 |
G2 | 191 | 550 | Leaf | 12 | 23.9 | 0.15 | |
GL2 | 148 | 420 | Leaf | 23 | 17.7 | 0.14 | |
GZ | 110 | 300 | Leaf | 9 | 12.8 | 0.15 | |
P. persica (225 Mb) |
PXLc | 11.1 | 21 | Leaf | 23 | 3.74 | 0.52 |
Rootd | 13 | 29.8 | 4.06 | ||||
HY2 | 14 | 25 | Leaf | 16 | 6.19 | 0.62 | |
Petal | 13 | 11.31 | 1.13 | ||||
NJAU1 | 15.9 | 30 | Leaf | 26 | 6.46 | 0.56 | |
NJAU2 | 37.6 | 50 | Leaf | 8 | 6.25 | 0.40 | |
Maoping | 12.8 | 40 | Leaf | 16 | 3.56 | 0.26 | |
DHQ1 | 3.1 | 2 | Leaf | 75 | 1.97 | 2.54 | |
P. mume (220 Mb) |
MHG1 | 17.5 | 20 | Leaf | 25 | 12.9 | 2.17 |
Rootd | 32 | 25.4 | 4.82 | ||||
MHG2 | 10.2 | 8 | Leaf | 33 | 5.7 | 2.38 | |
S. suchowensis (480 Mb) |
YAF1 | - | 1 | Leaf | 19 | 1.26 | 2.58 |
Rootd | 21 | 2.86 | 6.60 | ||||
B. distachyon (272 Mb) |
WD2 | - | 1 | Leaf | 29 | 3.17 | 6.13 |
Rootd | 8 | 4.75 | 8.97 | ||||
Lemma | 7 | 2.57 | 4.97 | ||||
Fragaria vesca (210 Mb) |
FH1 | - | 1 | Leaf | 45 | 1.93 | 6.37 |
Stemse | 4 | 4.75 | 15.78 | ||||
Arabidopsis thaliana (119 Mb) |
Col17+Col24 | - | 1 | Leaf | 64 | 0.69 | 4.35 |
Oryza sativa (373 Mb) |
KA1+PA1+ DG1+NIPB | - | 1 | Leaf (Tiller)f | 29 | 4.79 | 9.01 |
Leaf (Callus) | 13 | 194.8 | 287.1 |
aMutation rate per bp per year corrected for accessible genome regions.
bThe age of G1 was estimated to be at least 600 years old by comparing with another about 900-year-old peach tree whose diameter is about 240 cm, and this tree is only a few kilometers away from G1.
cThe age of the PXL peach tree was estimated to be about 21 years old through counting its annual ring in 2016.
dThese four samples contain both leaves from different trunk branches and roots from different underground root branches; in samples of tree roots, DNA was extracted from the phloem of the root.
eIn these strawberry samples, four runner regions in the same vine were chosen for sequencing (see Fig 3B for details).
fTiller samples from culture-derived plants were not included.