Fig 4. GSK3 inhibition decreases β-catenin phosphorylation and increases β-catenin levels in primary lung lymphatic endothelial cells.

Primary human lung lymphatic endothelial cells were treated with either SB216763 (1μM) or vehicle control (DMSO) for 48h. Equal amounts of protein from whole cells lysates were analyzed by western blotting with antibodies against phosphorylated and total β-catenin and β-actin (A). Ratio of phosphorylated and total β-catenin (B), and total β-catenin/β-actin (C) expressed as fold change relative to control. Data represent means ± SEM of three independent experiments. **P <0.01 by t-test.