Table 1. HIV-1 PCR primers.
| Set | Primer | Sequence (5' - 3') | Position (nt)a | Annealing Temperature (°C) | Product size (nt)a |
|---|---|---|---|---|---|
| Protease | |||||
| 1 | HIV-1_PR-2F | CACCAAATGAAAGATTGTACTGAG | 2050–2073 | 2F/2R: 56 | 678 |
| HIV-1_PR-2R | CTGGAGTATTGTATGGATTTTCAGG | 2703–2727 | |||
| 2 | HIV-1_PR-4F | GAGGCAATTTTAGGAACCAAAG | 1928–1949 | 4F/4R: 55 | 883 |
| HIV-1_PR-4R | GAAGTCTTGAGTTCTCTTATTAAGTT | 2785–2810 | |||
| Reverse Transcriptase | |||||
| 1 | HIV-1_RT-1F | TAAAAGCATTAGTAGAAATTTGTAC | 2641–2665 | 1F/1R: 56 | 622 |
| HIV-1_RT-1R | TATCAGGATGGAGTTCATAACC | 3240–3261 | |||
| 2 | HIV-1_RT-2F | GTTAAACAATGGCCATTGACAG | 2610–2632 | 2F/1R: 56 | 652 |
| HIV-1_RT-1R | TATCAGGATGGAGTTCATAACC | 3240–3261 | |||
| Integrase | |||||
| 1 | HIV-1_IN-2F | GTGCTGGAATCAGGAAAGTACT | 4207–4228 | 2F/2R: 53 | 967 |
| HIV-1_IN-2R | CATAGTGATGTCTATAAAACCATCC | 5149–5173 | |||
| 2 | HIV-1_IN-5F | GGAATTGGAGGAAATGAACAAGTAG | 4170–4194 | 5F/5R: 53 | 997 |
| HIV-1_IN-5R | ATGTCTATAAAACCATCCCCTAGC | 5143–5166 | |||
| 3 | HIV-1_IN-3771F | GCCACCTGGATTCCTGAGTG | 3771–3790 | 3771F/5266R: 53 | 1496 |
| HIV-1_IN-5266R | CTCCTGTATGCAGACCCCAATAT | 5244–5266 | |||
| 4 | HIV-1_IN-4074F | CAACCAGATAAAAGTGAATCAG | 4074–4095 | 4074F/5266R: 53 | 1193 |
| HIV-1_IN-5266R | CTCCTGTATGCAGACCCCAATAT | 5244–5266 | |||
a According to HIV-1 reference strain HXB2 (GeneBank accession number K03455). nt, Nucleotides.