Table 2. O2 flux of permeabilised fibres under different respiratory states.
| Assay One | CON | MCT | MCT + BB | p ≤ 0.05 |
| CI + CII OXPHOS (ADP) (pmols s-1 mg-1) | 233 ± 10 | 191 ± 12 | 182 ± 12 | * # |
| CI / CI + CII OXPHOS (ADP) | 0.51 ± 0.06 | 0.34 ± 0.02 | 0.35 ± 0.03 | * # |
| Assay Two | CON | MCT | MCT + BB | p ≤ 0.05 |
| CI + CII ADP-limited OXPHOS (ATP) (pmols s-1 mg-1) | 207 ± 9 | 198 ± 24 | 141 ± 7 | # p |
| CI / CI + CII ADP-limited OXPHOS (ATP) | 0.59 ± 0.01 | 0.52 ± 0.03 | 0.54 ± 0.02 | |
| ET (pmols s-1 mg-1) | 294 ± 18 | 242 ± 32 | 200 ± 17 | # |
Mean ± SEM steady-state respiratory states (pmol s-1 mg-1) from control (CON; n = 6), monocrotaline (MCT; n = 5) and metoprolol treated MCT (MCT + BB; n = 6) permeabilised RV fibres. Respiration was measured with combined CI (glutamate, malate and pyruvate) and CII (succinate) substrates stimulated with either ATP (ADP-limited OXPHOS) or ADP (OXPHOS). Fractional CI acitivity was determined by normalising CI OXPHOS O2 flux to combined CI + CII OXPHOS (CI / CI + CII). The maximum ETS flux (ET) was measured following uncoupling with FCCP (MCT; n = 4).
* p ≤ 0.05 for CON vs. MCT and
# for CON vs. MCT + BB and Ɨ for MCT vs. MCT + BB using an ordinary one-way ANOVA with Sidak’s multiple comparisons.