Extended Data Fig. 6 |. Comparison of cell types to those defined previously20.

We compared the similarity of clustering results for our current dataset and our previous dataset by nearest centroid classification (Methods). We mapped the 1,424 cells from the previous dataset²⁰ to 133 clusters from the current study (a) and vice versa: 23,822 current cells to 49 previous clusters²⁰. b, Some types were largely absent from our previous study. For example, the Meis2 type was not detected probably owing to rarity and white-matter confinement, whereas the L5 near-projecting types were missed owing to reliance on Rbp4-cre_KL100 to isolate L5 cells. We now find that, in contrast to pan-neuronal (Snap25-IRES-cre) or pan-glutamatergic Cre lines (Slc17a7-IRES-cre), Rbp4-cre_KL100 labels all L5 types except L5 near-projecting (Extended Data Fig. 8).