Extended Data Fig. 15 |. Cell types with activity-dependent transcriptomic signatures.

a, River plots representing the mapping of 14,205 VISp cells from this study to a previously published dataset⁴⁰ using a centroid classifier (Methods). b, Violin plots show the distribution of log₂-scaled and centred average expression of late-response genes (LRGs) and early-response genes (ERGs) in select cell types from this study that are in a subclass with at least one type that is significantly correlated with LRG or ERG expression. From the published dataset⁴⁰, only the clusters with expression of at least four ERGs and LRGs were included. In total, values for n = 6,956 cells from our study are displayed in the violin plots. We performed a two-sided t-test to assess enrichment or depletion of expression of LRGs or ERGs, and defined significant values as P < 0.01 after correction for multiple hypotheses using the Holm method, and average fold change greater than 2. Significant P values for enrichment and depletion are displayed above and below each row, respectively. Complete statistics for all t-tests is included in Supplementary Table 11. c, Heat maps of log₂-scaled and centred average gene expression for ERGs and LRGs in select cell types from our study and from the published dataset⁴⁰.