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. 2019 Mar 11;8:e41223. doi: 10.7554/eLife.41223

Figure 2. rEPN neurons innervate LHb neurons projecting to the RMTg.

(A, B) Schematics of procedure in which retrogradely transported virus expressing Cre-recombinase was injected into the RMTg or VTA and viruses expressing flox-stopped TVA and RG were injected into the LHb. EnvA-ΔG-rabies virus was injected into the LHb 3 weeks later. Animals were perfused at the end of the forth week. (C) Neurons in the rEPN and the VTA preferentially projected to RMTg-projecting LHb neurons over VTA-projecting LHb neurons. (D) Representative pictures of RMTg-projecting TVA expressing cells and first-order rabies infected cells in the LHb. (E) Representative pictures of second-order rabies infected cells in the VP, LPO, rEPN, LH, and VTA after Cav2-Cre injection into RMTg, indicating neurons disynaptically connected to RMTg.

Figure 2.

Figure 2—figure supplement 1. Representative images of neurons innervating VTA-projecting LHb neurons.

Figure 2—figure supplement 1.

(A) Schematics of procedure for viral tracer experiments. (B) Representative pictures of VTA-projecting TVA expressing cells (red label) and first-order rabies infected cells (green label) in the LHb. (C–F) Representative pictures of second-order rabies infected cells in the VP, LPO, rEPN, LH, and VTA after Cav2-Cre injection into VTA.