Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Escherichia coli) | mrcB | NA | EcoCyc:EG10605 | |
| Gene (E. coli) | mrcA | NA | EcoCyc:EG10748 | |
| Gene (E. coli) | lpoA | NA | EcoCyc:G7642 | |
| Gene (E. coli) | lpoB | NA | EcoCyc:G6565 | |
| Strain, strain background (E. coli) |
MG1655; wild-type | PMID:6271456 | ||
| Strain, strain background (E. coli) |
UTI89 | PMID:11402001 | ||
| Antibody | anti-PBP1a | Gift of Waldemar Vollmer | (1:5000) | |
| Antibody | anti-PBP1b | Gift of Waldemar Vollmer | (1:1000) | |
| Antibody | anti-FtsZ | Gift of David Weiss | (1:5000) | |
| Recombinant DNA reagent | mrcB::kan; ∆mrcB | PMID:16738554; Coli Genetic Stock Center |
CGSC:JW0145-1 | |
| Recombinant DNA reagent | mrcA::kan; ∆mrcA | PMID:16738554; Coli Genetic Stock Center |
CGSC:JW3359-1 | |
| Recombinant DNA reagent | lpoA::kan; ∆lpoA | PMID:16738554; Coli Genetic Stock Center |
CGSC:JW3116-1 | |
| Recombinant DNA reagent | lpoB::kan; ∆lpoB | PMID:16738554; Coli Genetic Stock Center |
CGSC:JW5157-1 | |
| Recombinant DNA reagent | pCP20 | PMID:10829079 | ||
| Recombinant DNA reagent | pUM1Bα | PMID:12949085 | ||
| Recombinant DNA reagent | pUM1Bγ | PMID:12949085 | ||
| Recombinant DNA reagent | pUM1Bα* | PMID:12949085 | ||
| Recombinant DNA reagent | pUM1BTG*α | PMID:12949085 | ||
| Recombinant DNA reagent | pUM1BTG*α* | PMID:12949085 | ||
| Chemical compound, drug | Ampicillin Sodium Salt | Sigma Aldrich | Catalog:A9518 | |
| Chemical compound, drug | Amoxicillin | Sigma Aldrich | Catalog:A8523 | |
| Chemical compound, drug | Cefsulodin Sodium Salt Hydrate | Sigma Aldrich | Catalog:C8145 | |
| Chemical compound, drug | Mecillinam | Sigma Aldrich | Catalog:33447 | |
| Chemical compound, drug | Doripenem Hydrate | Sigma Aldrich | Catalog:SML1220 | |
| Chemical compound, drug | Meropenem Hydrate | Sigma Aldrich | Catalog:M2574 | |
| Chemical compound, drug | Cephalexin | Sigma Aldrich | Catalog:33989 | |
| Chemical compound, drug | Aztreonam | Sigma Aldrich | Catalog:A6848 | |
| Chemical compound, drug | Piperacillin Sodium Salt | Sigma Aldrich | Catalog:P8396 | |
| Software, algorithm | SuperSegger | PMID:27569113 | ||
| Software, algorithm | FIJI | PMID:22743772 | ||
| Other | Propidium iodide | Sigma Aldrich | Catalog:81845 |