Figure 3.

Loci detected across GPs and temperature sensitivity classes. Loci interacting with GPa and GPb backcross segregants at 21 and 30° act in specific allele combinations. As shown with black lines, certain temperature-sensitive genotypes provide the potential for additional alleles to eliminate temperature sensitivity. These additional alleles are designated with bold outlines. In the legend, “mutant” refers to the de novo or induced mutations present involved in each GP. Additionally, the prFLO11 recombination (GPb) is indicated with a red outline. Note, the number of loci that influence the trait differs significantly among the three GPs, with GPa and GPc showing the lowest and highest genetic complexity, respectively. 3S, derivative of clinical isolate 322134S; BY, laboratory strain BY4716; GP, genetic perturbation.