Table 2. Summary of one-shot Nested CRISPR experiments.
| Experiment | Plates with dpy-10 edits/injected worms | Step 1 positives/worms screened (%)a | Step 2 positives/worms screened (%)a |
|---|---|---|---|
| prpf-4::mCherry | 11/29 | 25/38 (65.79) | 16/77 (20.78)b |
| gtbp-1::EGFP | 6/28 | 3/40 (7.50) | 0/40 (0) |
| pgl-1::EGFP | 2/22 | 4/25 (16.00) | 1/25 (4.00) |
| F27C1.2::wrmScarlet | 7/22 | 7/40 (17.50) | 7/144 (4.86)b |
| EGFP::nfki-1 | 5/26 | 28/70 (40.00) | 0/70 (0) |
a
Based on PCR genotyping (amplicons of the correct size are considered positives regardless of whether or not the insertion is in-frame).
b
Identification of positives via visual screening.