Table 1. Germline and vulval phenotypes in adsl-1(RNAi) and adss-1(RNAi).
| Genotype (+pie-1p::H2B::GFP) | Sterile no germline (%) | Sterile <10 germ cells (%) | Sterile >10 germ cells (%) | WT-like germline (%) | N = (germline) | Protruding vulva (%) | WT-like vulva (%) | N = (vulva) |
|---|---|---|---|---|---|---|---|---|
| WT | 0 | 0 | 2.3 | 97.7 | 43 | 0 | 100 | 35 |
| ppat-1(Δ) | 0 | 0 | 0 | 100 | 57% | 0 | 100 | 57 |
| adsl-1(RNAi) | 8.1 | 0 | 51.4 | 40.5 | 37 | 26.1 | 73.9 | 23 |
| ppat-1(Δ); adsl-1(RNAi) | 3.4 | 0 | 27.6 | 69.0 | 29 | 0 | 100 | 28 |
| adss-1(RNAi) | 1.7 | 1.7 | 57.9 | 38.8 | 121 | 8.0 | 92.0 | 50 |
| ppat-1(Δ); adss-1(RNAi) | 0 | 0 | 7.7 | 92.3 | 52 | 0 | 100 | 39 |
All strains carry the germline specific pie-1p::H2B:GFP. Three classes of sterile phenotypes were defined based on the number of GFP positive germline nuclei. Vulval phenotypes were scored on DIC (Nomarski) microscopy.