Skip to main content
. 2019 Jan 30;211(4):1315–1330. doi: 10.1534/genetics.119.301970

Figure 2.

Figure 2

Cis-regulatory lesions in Oti-lin-3/EGF cause hypoinduction of primary (1°) and secondary (2°) vulval cell fates. (A) P(4–8) cell fates in the wild-type CEW1 O. tipulae reference strain and mutants for lin-3/EGF. The pie diagrams represent the percentage of cell fates over individuals. Yellow, red, and blue are for the tertiary (3°), 2°, and 1° fates, respectively. Gray denotes an undivided cell fused to the hypodermis. The Vulva Index (V.I.) is calculated as the average number of cells acquiring a vulval cell fate in a set of animals. The quantifications of Oti-lin-3(mf86) are from Dichtel-Danjoy and Félix (2004b). (B) Position of the deletions in the trimethylpsoralene-ultraviolet (TMP-UV) and clustered regularly interspaced short palindromic repeat (CRISPR) alleles. As in C. elegans, the lin-3 gene of O. tipulae is predicted to have two alternative ATGs, with the anchor cell cis-regulatory element upstream of the second ATG. However, unlike C. elegans, O. tipulae has a single E-box “CACCTG” binding site and no nuclear hormone receptor (NHR) binding site. Note that the seven exons following the second ATG were excluded from the diagram. (C) Distributions of Oti-lin-3 mRNA number in the anchor cell of wild-type CEW1 and lin-3 cis-regulatory mutants, as quantified by single-molecule FISH. n.s., not significant. ***: p<0.001.