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. Author manuscript; available in PMC: 2020 Jan 9.
Published in final edited form as: Cell Host Microbe. 2019 Jan 9;25(1):73–86.e5. doi: 10.1016/j.chom.2018.12.008

Figure 3: Nef impairs stabilization of cell polarity in HIV-infected T cells.

Figure 3:

(A) ORF diagrams of indicated HIV reporter strains. To produce virus capable of one round of infection, a complementary helper plasmid encoding intact env was used for viral packaging.

(B) Experimental protocol

(C) Migratory tracks,

(D) Mean track velocities,

(E) Arrest coefficients and

(F) MD plots of HIV-infected cells. Blue lines and numbers indicate medians. MC = motility coefficient (in ×m × min−1).

(G) MP-IVM time-lapse series of representative T cells infected with env-deficient HIV expressing Nef (red) or not (green). Elapsed time in minutes:seconds.

(H) Circularity index over time for cells shown in (G). Circles correspond to measurements of cells at various time points depicted in (G).

(I) Mean track circularities.

Data are pooled from 6 individual recordings from 2 animals. MC = motility coefficient (in μm × min−1). n.s. = not significant. Blue lines and numbers above graphs indicate medians. n.s. = not significant.