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. Author manuscript; available in PMC: 2019 Apr 10.
Published in final edited form as: J Cell Biochem. 2015 Oct;116(10):2177–2187. doi: 10.1002/jcb.25089

Fig. 4.

Fig. 4.

Lyar selectively binds to apoptotic cells and shed POS vesicles, but not healthy cells. Lyar-FLAG was expressed in HEK293 (A) or Neuro-2a cells (B), followed by induction of apoptosis. Lyar binding to apoptotic cells was detected with anti-FLAG antibody, followed by Texas red-labeled secondary antibody. Apoptotic cells were labeled with FITC-annexin V and analyzed by fluorescence microscopy (A) or flow cytometry (B). Bar = 50 μm. (C) Lyar binds to POS vesicles. POS vesicles were incubated with Lyar-FLAG or GFP-FLAG lysate. After washing, POS-bound FLAG-tagged protein was detected by anti-FLAG mAb and HRP-labeled secondary antibody, followed by colorimetric assay (± SEM, n=5, t-test, ***P<0.001)