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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Fungal Genet Biol. 2019 Feb 6;126:17–24. doi: 10.1016/j.fgb.2019.02.002

Fig. 3.

Fig. 3.

FoSSO1 gene C-terminal tagging with a mCherry fluorescent marker based on the HDRI strategy. (A) An illustration of the homology-dependent recombination integration (HDRI) strategy for the C-terminal tagging of the FoSSO1 gene. A homologous upstream fragment was amplified prior to the gene stop codon and fused with mCherry, the hygromycin resistance cassette, and a homologous downstream fragment that is immediately after the sgRNA cleavage site. During transformation, two homologous recombination events are necessary to occur and the mCherry-Tef-1-HYGB fragment will be integrated at the 3′-terminus of the endogenous gene. (B) the FoSso1-mCherry subcellular localization in different fungal structures. a and b: conidia, c and d: germlings, e and f: fungal tips and hyphae. Gradient red arrows weaken from strong to weak, indicating the different distribution of the FoSso1 protein in relation to the fungal hyphal tip. Scale bars, 10 μm.