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. Author manuscript; available in PMC: 2019 Aug 2.
Published in final edited form as: Cell Stem Cell. 2018 Jul 12;23(2):226–238.e4. doi: 10.1016/j.stem.2018.06.005

Figure 5. Pluripotency transcription factors are enriched at alternative ESC enhancers near GRHL2 targets.

Figure 5.

A) Motif analysis at the alternative enhancers for GRHL2 targets in ESCs to identify candidate TF motifs that are enriched at these sites. B) Pie chart showing percentage of GRHL2 target genes in EpiLCs that are nearest to a KLF4-bound site in ESCs. KLF4 binding sites at promoters were excluded to enrich for enhancers. C) Heatmaps for enhancer marks in ESCs and EpiLCs at ESC KLF4 sites that have a GRHL2 target as its nearest gene. All plots are shown for an 8kb window centered at KLF4 binding sites. D) Metagene analysis showing average signal for the heatmaps in (C). A significant decrease in signal in the EpiLC state was observed for each enhancer mark except for ATAC-seq signal. (K4me1 p = 2.81E-3; K27ac p = 4.83E-12; SMC1 p = 1.87E-10).