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. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: Dev Cell. 2019 Feb 28;49(1):89–99.e4. doi: 10.1016/j.devcel.2019.02.001

Figure 3. Microbial Colonization of the Intestine Leads to Enhanced Microbial Aversion Behavior.

Figure 3.

(A) Colony forming units per animal for N2 animals grown on control as well as aex-5, flr-1, nhx-2, and pbo-1 RNAi followed by exposure to P. aeruginosa-GFP for 5 hours at 25°C. EV, empty vector RNAi control. ***p < 0.001, **p < 0.01, and *p < 0.05 via the t-test.

(B)-(D) Percent occupancy of P. aeruginosa (B), Serratia marcescens (C), and P. aeruginosa gacA mutant (D) lawns over time by N2 animals grown on control as well as aex-5, flr-1, nhx-2, and pbo-1 RNAi. EV, empty vector RNAi control.

(E) Percent occupancy of P. aeruginosa lawns over time by N2 animals grown on S. enterica for 48 hours post L4 larval stage. The control animals were grown on E. coli.

(F) Aversion to E. coli HT115 upon knockdown of aex-5, flr-1, nhx-2, and pbo-1 by RNAi. ***p < 0.001 and **p < 0.01 via the t-test.

See also Figures S2 and S3.