Skip to main content
. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: Dev Cell. 2019 Feb 28;49(1):89–99.e4. doi: 10.1016/j.devcel.2019.02.001

Figure 4. Intestinal Lumen Bloating Activates Innate Immune Genes.

Figure 4.

(A) Representative photomicrographs of clec-60p::GFP animals grown on RNAi control bacteria as well as bacteria for RNAi against aex-5, flr-1, nhx-2, and pbo-1. EV, empty vector RNAi control.

(B)-(H) Quantitative reverse transcription-PCR (qRT-PCR) for the immune genes clec-60 (B), cpr-2 (C), F53A9.8 (D), ilys-3 (E), lys-4 (F), lys-5 (G), and ugt-18 (H) in N2 animals grown on control as well as aex-5, flr-1, nhx-2, and pbo-1 RNAi. EV, empty vector RNAi control. ***p < 0.001, **p < 0.01, and *p < 0.05 via the t-test. n.s., non-significant.

(I) Representative photomicrographs of N2 animals grown on live E. coli until the young adult stage, followed by incubation on HK E. coli for 24 hours at 20°C. The control animals were maintained on live E. coli. Representative photomicrographs of the head and tail regions are shown. Arrows point to the border of the intestinal lumen.

(J) Gene expression levels for the immune genes in N2 animals grown on live as well as HK E. coli. The animals were grown on live E. coli until the young adult stage before incubation on HK E. coli for 24 hours at 20°C. ***p < 0.001, **p < 0.01, and *p < 0.05 via the t-test.

See also Figures S4 and S5.