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. 2019 Mar 15;11(3):1521–1530.

Figure 4.

Figure 4

NEDD4 co-expression enhances the stability of KLF8. A. The half-life of KLF8 protein is prolonged by NEDD4. HA-KLF8 was co-expressed with an empty vector (-NEDD4) or Myc-NEDD4 (+NEDD4) in HEK293 cells for 48 hours. The cells were treated with 50 mg/ml cycloheximide for various times indicated. Whole cell lysates were prepared at each of the time points for IB with an anti-HA antibody with the b-actin loading control. B. The chemiluminescence intensity of KLF8 protein in the blots was quantified using Image Lab 3.0 (Bio-Rad, Hercules, CA) as we previously reported [12] and graphed in relative to the zero point of time. *P<0.005.