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NEDD4 regulates KLF8 likely in the nucleus. HEK293 cells were transfected with the plasmids encoding HA-KLF8, wild type (WT) or mutants (S48A or S48D), alone (A) or together with the plasmid encoding Myc-NEDD4 (B). After 24 hours, the cells were fixed, permeabilized, and stained with an antibody for HA or Myc and the Hoechst dye for the nucleus followed by fluorescein-conjugated secondary antibody, and photographed by fluorescent microscopy.
