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IL-27 concentrations less than 200 ng/ml did not affect cell proliferation after 24 h, 48 h and 72 h. RAW264.7 cells were treated with different concentrations of IL-27 (0, 50, 100, 200 ng/ml) for 24 h, 48 h and 72 h. Cell viability was assessed using a Cell Counting Kit-8 (CCK-8) assay. (*P < 0.05, **P < 0.01).
