Figure 4.

IL-27 suppresses osteoclast differentiation and marker gene expression by inhibiting the NF-κB signaling pathway. A. RAW264.7 cells were pretreated with or without IL-27 for 2 h in the presence of M-CSF (25 ng/ml) before RANKL (100 ng/ml) stimulation for the indicated time. B. Cells were lysed for Western blot analyses using antibodies against NFATc1, C-FOS, and β-actin. C-K. The expression of genes associated with osteoclast differentiation, fusion, and function were detected by qRT-PCR. (*P < 0.05, **P < 0.01).