Figure 5.

IL-27 downregulates LPS-induced osteoclastogenesis and reduces the expression of osteoclast marker genes by reducing pro-inflammatory cytokines. A. TRAP staining was used to analyze LPS-induced osteoclast differentiation. B. TRAP-positive osteoclasts (nuclei ≥ 3) in each well were quantitatively analyzed. C-K. The expression of genes associated with osteoclast differentiation, fusion, and function was detected by qRT-PCR. L. Cells were lysed for Western blot analyses using antibodies against NFATc1, C-FOS, and β-actin. M-O. The mRNA expression of TNF-α, IL-1β, and IL-6 was detected by qRT-PCR. (*P < 0.05, **P < 0.01).