Table 1.
Primer sequences for qRT-PCR
| Genes | Forward | Reverse |
|---|---|---|
| NFATc1 | 5’-GACCCGGAGTTCGACTTCG-3’ | 5’-TGACACTAGGGGACACATAACTG-3’ |
| C-FOS | 5’-CGGGTTTCAACGCCGACTA-3’ | 5’-TTGGCACTAGAGACGGACAGA-3’ |
| CTSK | 5’-GAAGAAGACTCACCAGAAGCAG-3’ | 5’-TCCAGGTTATGGGCAGAGATT-3’ |
| TRAP | 5’-CACTCCCACCCTGAGATTTGT-3’ | 5’-CATCGTCTGCACGGTTCTG-3’ |
| DC-STAMP | 5’-CTAGCTGGCTGGACTTCATCC-3’ | 5’-TCATGCTGTCTAGGAGACCTC-3’ |
| OC-STAMP | 5’-GGGCTACTGGCATTGCTCTTAGT-3’ | 5’-CCAGAACCTTATATGAGGCGTCA-3’ |
| CTR | 5’-CGCATCCGCTTGAATGTG-3’ | 5’-TC TGTCTTTCCCCAGGAAATGA-3’ |
| MMP9 | 5’-CTGGACAGCCAGACACTAAAG-3’ | 5’-CTCGCGGCAAGTCTTCAGAG-3’ |
| ATP6V0d2 | 5’-GATGGAGAAGCTGATGGCTTGG-3’ | 5’-TTCTTCACCTCGCCTGTCTTGC-3’ |
| IL-1β | 5’-CTCAACTGTGAAATGCCACC-3’ | 5’-TGTCCTCATCCTGGAAGGT-z’ |
| IL-6 | 5’-TGGGAAATCGTGGAAATGAGA-3’ | 5’-ACTCTGGCTTTGTCTTTCTTGT-3’ |
| TNF-α | 5’-AGGCGGTGCTTGTTCCTCA-3’ | 5’-AGGCGAGAAGATGATCTGACTGC-3’ |
| GAPDH | 5’-AAATGGTGAAGGTCGGTGTG-3’ | 5’-TGAAGGGGTCGTTGATGG-3’ |
Note. NFATc1: nuclear factor of activated T cells; CTSK: cathepsin K; TRAP: tartrate-resistant acid phosphatase; DC-STAMP: dendritic cell-specific transmembrane protein; CTR: calcitonin receptor; MMP9: matrix metalloproteinase-9; IL-1β: interleukin-1β; IL-6: interleukin-6; TNF-α, tumor necrosis factor-α; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; qRT-PCR, quantitative real-time polymerase chain reaction.