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3D human liver microtissue NASH model with severe fibrotic phenotype. The microtissues (MTs) were treated with 0.5 mM palmitic acid (PA) in the presence or absence of 1 uM of Selonsertib or with Medium+DMSO on day 0 and day 2. On day 9 all the MTs and supernatants were collected and analyzed for various endpoints.
