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Overexpression of Wnt4 in LPS-treated DPSCs promoted odontogenic differentiation. A. The MOI and transfection efficiency of the lentiviral vector used for Wnt4 overexpression in LPS-induced DPSCs (LPS-DPSCs). B. ALP staining of LPS-DPSCs with upregulated Wnt4 expression. C. ARS staining of LPS-DPSCs with upregulated Wnt4 expression. D. Relative expression of Runx2, IBSP, DSPP and COL1A1 in LPS-DPSCs overexpressing Wnt4. ***P<0.001.
