Figure 3.

Effect of Retinol palmitate pretreatment on I/R-induced apoptosis in H9c2 cells. A. Typical images of flow cytometry. B. The apoptosis ratio was quantified by BD FACS software. Retinol palmitate pretreatment significantly reduced apoptosis compared with the IR group in a dose-dependent manner. I/R: ischemia/reperfusion. DMSO: I/R+0.1% DMSO. I/R+L: IR+0.1 μM retinol palmitate. I/R+H: I/R+1 μM retinol palmitate. Data are expressed as mean ± SD (n = 6 per group). *P < 0.05, IR group and DMSO group versus control group. #P < 0.05, Retinol palmitate group versus IR group.