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. 2019 Apr 9;9:5838. doi: 10.1038/s41598-019-42084-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of differentially expressed genes in gpa1-5gcr1-5 double mutant. A total of 19 DEGs (10 up- and 9 down-regulated) were selected and subjected to RT-qPCR. The experiment was carried out using three biological replicates and the values are presented as log₂FC ± SE. qPCR was performed in triplicate and the ratios of statistics were calculated relative to the internal control gene Actin2 (*P < 0.05, **P < 0.01 vs. control).