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. 2019 Apr 9;9:5810. doi: 10.1038/s41598-019-42118-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scatter plot of the features detected (intensity >1.0E4, +/−0.005 m/z) in a single soil water extract and the elution profiles for HILIC (top) and RP (mirrored bottom) in positive-ion mode demonstrating different separation profiles of LMW DOM on each LC phase. Each marker matches to a m/z and retention time (RT). The corresponding normalized base peak chromatograms are overlaid on top to show a typical elution profile for each LC condition and display trends between m/z and RT. Additional examples in negative-ion mode are shown in Fig. S4.