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. 2019 Apr 9;9:5800. doi: 10.1038/s41598-019-41701-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative photomicrographs show immunostaining of MEDAG in different subtypes of PTC. Strong cytoplasmic staining of MEDAG in the classical PTMC with a detectable BRAFv600 mutation (A), (C) and (D). Strong nuclear staining of MEDAG in the follicular variant of PTMC without a detectable BRAFv600 mutation (E), (G) and (H). Staining of MEDAG was limited to the margin in tall cell variants PTMC. The black arrow indicates the high expression in the cytoplasm (I), (K) and (L) compared to PPARG (red fluorescent in L). Nuclear staining by DAPI (blue fluorescent in B, F and J). MEDAG (green fluorescent in C, D, G, H, K and L). Original magnifications: E × 100; A–D, F–L × 400.