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. 2019 Apr 9;9:5817. doi: 10.1038/s41598-019-42290-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tube-opening behaviour test for both cagemate and stranger mice, and tube-opening behaviour test for anesthetised cagemate mice. (A) Schematic diagram of the test. Tube-opening behaviour test for cagemate and stranger mice: time spent in the area (B) and latency to lid-opening (C). (D) Individual latency to lid-opening in the tube-opening behaviour test for both cagemate and stranger mice. (E) Schematic diagram of this test. (F) Tube-opening behaviour test for anesthetised cagemate mice: time spent in the anesthetised cagemate area or empty area. (G) Comparison of the latency to lid-opening for an anesthetised and a non-anesthetised mouse. All data are presented as box plots. The p values were calculated using paired t-test (B,F), Wilcoxon signed-rank test (C), and one-way repeated measures ANOVA (G). n = 9 animals per test.