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. 2019 Apr 9;9:5817. doi: 10.1038/s41598-019-42290-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tube-opening behaviour test under aversive conditions. (A) Schematic diagram of this test. Half of the home cage contains wet bedding and the other half contains dry, normal bedding. Tube containing the cagemate mouse on the wet bedding. (B) Tube-opening behaviour test for cagemate under the aversive condition: time spent in the area. (C) Average speed of the test mouse in each bedding. (D) Comparison of the latency to lid-opening when the tube contains the cagemate is placed on wet or normal bedding. (E) Latency to lid-opening in various conditions of the tube-opening behaviour test. All data are presented as box plots. (E) Statistical significance is represented by top bars: *p < 0.05. The p values were calculated using paired t-test (B,C) and one-way repeated measures ANOVA (D,E). n = 9 animals per test.