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. 2019 Apr 9;9:5817. doi: 10.1038/s41598-019-42290-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Preference tests for constrained cagemate and non-constrained cagemate mice in the social interaction test apparatus. (B) Schematic diagram of the apparatus of this experiment. Two transparent cages [(a), and (b)] are placed at both ends of a rectangular apparatus, and half of the area of the apparatus is taken as the respective area [area (a) and area (b)]. A radius of 20 cm around the transparent cage was set around the cage [around cage (a) and around cage (b)]. (B) Sample picture of the transparent cages containing constrained and non-constrained cagemates. (C) Test schedule. For each mouse, four tests were conducted according to the contents of the table. Cagemates in each state were placed in transparent cages (a) and (b). Preference tests for constrained cagemate and intact mice: time spent around the cage (D), and preference index defined as (time spent around cage (a))/(time spent around cage (a) + time spent around cage (b)). All data are presented as box plots. The p values were calculated using Wilcoxon signed-rank test (D) and one-way ANOVA (E). n = 16 animals per trial.