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rSK1-KD 3.6 reduces rSK1-YFP expression in HEK293T cells. Western blot of total cell lysates of HEK293T cells transfected with rSK1-YFP and co-transfected with pcDNA3 or plldsRed or three different knock-down hairpin constructs: rSK1-KD 2.4 or rSK1-KD 3.6 or rSK1-KD 4.5. rSK1-KD 3.6 reduced the expression of rSK1-YFP by over 90% and was used in all further experiments. β-actin was used as a loading control.
