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. 2019 Apr 3;10:652. doi: 10.3389/fmicb.2019.00652

FIGURE 2.

FIGURE 2

Effect on the expression of defense related gene and activities of defense-related enzymes in the process of controlling watermelon Fusarium wilt by B. velezensis F21. (A,B) Expression of defense related gene in watermelon root in response to interaction between B. velezensis F21 and F. oxysporum f. sp. niveum. Time course of expression of JA signaling pathway marker gene Cla011143 (A) and SA signaling pathway marker gene Cla005426 (B) in the root of watermelon treated with B. velezensis F21and challenged with Fon. The expression values of the individual genes were normalized using Actin gene as an internal standard. (C–E) The activities of defense-related enzymes (CAT, POD, and SOD) in the root of watermelon pretreated with B. velezensis F21 and challenged with Fon. All data were presented as means of three replicates ± SD, and error bars represent SD for three replicates. Means with asterisk have significant differences (∗P < 0.05 and ∗∗P < 0.01; LSD test). All experiments were performed three times, and similar results were obtained.