Figure 7.

Primary and expanded HCMV-specific CD4+ T cells degranulate in response to antigen loaded NK and moDC. TNFα and IFNγ production concomitant to CD107a mobilization was monitored by flow cytometry in co-culture experiments combining HCMV-loaded primary NK cells or moDC with primary autologous CD4+ T cells (A,C,E) or, alternatively, antigen loaded expanded NK cells and HCMV-expanded CD4+ T cells (B,D,E). (A,B) Dot plots displaying the proportions of IFNγ+ CD107a+ CD4+ T cells in the primary (A) and expanded (B) experimental systems. Data from a representative experiment. (C,D) Proportions of TNFα+, IFNγ+, and CD107+ CD4+ T cells in the different experimental systems assayed (mean+SEM; n = 4). (E) Pie charts showing the distribution of CD4+ T cells that produce cytokines and degranulate against HCMV-loaded APC in the indicated experimental systems (mean; n = 4).