Table 2.
Measured hydrodynamic and structural parameters from MALS, AUC and SAXS compared with calculated hydrodynamic and structural parameters from the EM 3D reconstruction and SAXS models.
| BMPER | N-BMPER | ||
|---|---|---|---|
| Measured with: | |||
| MALS | Rh (Å) | 51 | 40 |
| MW (kDa) | 100.4 | 48.3 | |
| AUC | Rh (Å) | 53 | 41 |
| f/f0 | 1.73 | 1.74 | |
| Sw,20 | 4.59 | 2.92 | |
| SAXS | Rg (Å) | 48.0 | 33.2 |
| Dmax (Å) | 160 | 111 | |
| Volume (Å3) | 320,000 | 140,000 | |
| Model resolution (Å) | 44 (± 3) | 35 (± 3) | |
| Calculated from: | |||
| EM | Rh (Å) | 54.79 | N/A |
| Sw,20 | 4.09 | N/A | |
| Rg (Å) | 49.39 | N/A | |
| Dmax (Å) | 166.3 | N/A | |
| SAXS | Rh (Å) | 48.8 | 36.5 |
| f/f0 | 1.62 | 1.54 | |
| Sw,20 | 4.89 | 3.09 | |