Fig. 3.

Boxplots of ARI for 10 clustering methods across 100 simulations using Splatter. a Investigating how different levels of group effect affect clustering results. We set the mean parameters of three cell types as (0.20, 0.21, 0.22), (0.20, 0.22, 0.24), and (0.20, 0.24, 0.28) to represent three levels (low, medium, and high) of group difference. b Investigating how different levels of batch effect affect clustering results. We set the mean parameters of the five individuals as (0.1, 0.1, 0.1, 0.1, 0.1), (0.12, 0.12, 0.12, 0.12, 0.12), and (0.14, 0.14, 0.14, 0.14, 0.14) to represent three levels (low, medium, and high) of batch effects. In the boxplots, the box spans from the first to third quartile (depicting median as a line in the middle), the whiskers extend to 1.5× IQR (interquartile range)