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. 2019 Mar 11;9(4):e01254. doi: 10.1002/brb3.1254

Figure 6.

Figure 6

Nucleotide‐binding oligomerization domain‐like receptor with a pyrin domain 3 (NLRP3) knockdown reduces hemin‐induced cellular toxicity and degradation of zona occludens‐1 (ZO‐1), abolishing the protective effect of glibenclamide. (a) Flow cytometry analysis of cell apoptosis in the control, si‐NC, and si‐NLRP3 transfected b.End3 cells treated with or without glibenclamide. (b) Cell death was evaluated by the LDH release assay. (c) The apoptosis rate was plotted as a histogram. (d) Western blot analyses show that NLRP3 knockdown significantly increased hemin‐induced ZO‐1 degradation. (n = 3, *p < 0.05, **p < 0.01)