Squid colonization in group C strain SR5, which does not encode RscS, is dependent on the syp polysaccharide locus. Single-strain colonization experiments were conducted, and circles represent individual animals. The limit of detection for this assay, represented by the dashed line, is 7 CFU/light organ (LO), and the horizontal bars represent the median for each set. Hatchling squid were inoculated with 1.5 × 103 to 3.2 × 103 CFU/ml bacteria, washed at 3 h and 24 h, and assayed at 48 h. Strains are MJM1100, MJM3010, MJM3062, MJM1125, and MJM3501. Statistical comparisons were done with the Mann-Whitney test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.