FIG 6.

Group B and group A strains require the syp locus for robust squid colonization. The wild type (WT) and Δsyp derivatives of the indicated strains were assayed in a single-strain colonization assay. Hatchling squid were inoculated with 6.7 × 10² to 32 × 10² CFU/ml bacteria (ES114 and MB15A4 backgrounds) or 5.2 × 10² to 8.9 × 10² CFU/ml bacteria (MB11B1 and ES213 backgrounds), washed at 3 h and 24 h, and assayed at 48 h. Each dot represents an individual squid. The limit of detection is represented by the dashed line, and the bars represent the median for each set. Strains are MJM1100, MJM3062, MJM2114, MJM3071, MJM1130, MJM3065, MJM1117, and MJM3068. Statistical comparisons were done with the Mann-Whitney test. ****, P < 0.0001.