FIG 8.

MB11B1 sypE frameshift leads to an enhanced biofilm phenotype upon SypG overexpression. Shown are spot assays of strains carrying the pKV69 vector or pEAH73 SypG overexpression plasmid. (A) ES114 strain background. Strains lacking SypE produce a wrinkled colony phenotype upon SypG overexpression. Deletion of nucleotide 33 in sypE to mimic the group A frameshift led to earlier wrinkling and a more pronounced colony biofilm at 48 h. Strains are MJM1104, MJM3455, MJM3418, MJM3419, MJM3364, and MJM3365. (B) Group A strain MB11B1, which naturally carries a −1 frameshift in sypE, exhibits a cohesive phenotype at 48 h with overexpression of SypG. Deletion of sypE reduces this phenotype, and repairing the frameshift by addition of a guanosine at nucleotide 33 further reduces the cohesiveness of the spot. Strains are MJM3370, MJM3371, MJM3411, MJM3412, MJM3398, and MJM3399.