FIG 7.

T starvation at 42°C leads to the general destruction of even nonreplicating chromosomes. The strains are as follows: thyA mutant, KKW58; thyA dnaA601(Ts) mutant, SRK291. (A) Locations of the four chromosomal loci for which we probed: oriC, N-5, N-14, and dif (ter). (B) Evolution of the amounts of the four chromosomal loci (shown in panel A) in the thyA mutant during the T starvation awakening protocol at 42°C. (C) Same as for panel B but in the thyA dnaA(Ts) mutant. (D) TLD kinetics of the thyA mutant at 28°C, 37°C, or 42°C in the cultures used for analysis in panels E, F, and G. In contrast to all other data in the figures, the data in panels D, E, F, and G are from a single assay. (E to G) Evolution of the amounts of the four chromosomal loci, oriC, N-5, N-14, and dif (ter), in the thyA mutant during the standard T starvation protocol (growing cultures) at 28°C (E), 37°C (F), and 42°C (G). (H) General scheme of the nonreplicating chromosome changes during TLD.